Coding

Part:BBa_K3329047

Designed by: Wenfei Xiao   Group: iGEM20_BNDS_China   (2020-10-13)


Spider fibroin optimized V2

This part produces spider fibroin that can strengthen the abrasion resistance of the artificial leather.

It's noteworthy that, in our Protein Crosslinking design, we intended to adopt spider fibroin as the structural protein modification in order to enhance the tenacity of our artificial leather. Spider fibroin would be expressed in both P. Pastoris and E. coli, respectively. Therefore, codon optimization must be applied in order to avoid homologous recombination and fit the chassis organism. As a result, we synthesized 4 versions of genes coding the same spider fibroin sequence based on the 2 versions of codon optimization for P. Pastoris and 2 versions for the codon optimization of E. coli. Names for those 4 versions of CDS are documented from Spider fibroin optimized V1 to CBM optimized V4, in which the Spider fibroin optimized V1 and V2 are for P. Pastoris, and Spider fibroin optimized V3-4 are for E. coli.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 28


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